Thursday, 21 September, 2017
UT Host: Dr. Chris Baker, Assistant Chemistry Professor
Speaker: Dr. Susan Lunte
Ralph N. Adams Distinguished Professor of Chemistry and Pharmaceutical Chemistry
Director, Adams Institute for Bioanalytical Chemistry at The University of Kansas
Title: “Application of Lab-on-a-Chip Devices for In Vivo and In Vitro Monitoring of Biomarkers of Oxidative Stress”
Abstract: Microchip electrophoresis is a powerful tool for the analysis of biological samples. In particular, its ability to perform fast, efficient separations makes it possible to monitor several compounds simultaneously with high temporal resolution. The small dimensions of the channels in the chip are compatible with the analysis of microdialysis samples and single cells. In this presentation, two applications of microchip electrophoresis (ME) for biochemical investigations will be presented. The first involves the development of ME-based methods for the detection of reactive nitrogen and oxygen species (RNOS) in macrophages and immune cells. This includes direct amperometric detection of RNOS as well as the evaluation of fluorescent reagents used for specific species. ME allows detection of multiple substances in a single run, giving a better snapshot of the total RNOS production in the cells. The second application involves the combination of microdialysis with microchip electrophoresis for near real-time continuous monitoring of nitric oxide metabolites, catecholamines, and adenosine in awake, freely roaming animals. The goal of this application is to investigate the biochemical basis of neurodegenerative diseases caused by oxidative stress, such as epilepsy and traumatic brain injury.
Biography: Susan M. Lunte is the Ralph N. Adams Distinguished Professor of Chemistry and Pharmaceutical Chemistry, Director of the Adams Institute for Bioanalytical Chemistry, and Director of the NIH COBRE Center for Molecular Analysis of Disease Pathways at the University of Kansas, Lawrence, KS. She received a B.S. degree in chemistry from Kalamazoo College, Kalamazoo, MI, in 1980, and a Ph.D. in Analytical Chemistry in 1984 from Purdue University, West Lafayette, IN. At Purdue she worked with Peter Kissinger on using liquid chromatography with electrochemical detection to study the metabolism and toxicity of aromatic compounds. She then worked at Procter and Gamble for three years in the Food and Beverage division before joining the Center for Bioanalytical Research at the University of Kansas in 1987. She was appointed an associate professor of Pharmaceutical Chemistry at KU in 1995 and the Adams Professor of Chemistry and Pharmaceutical Chemistry in 2006.
Dr. Lunte has been the recipient of several awards over the years including a NSF CAREER Award, the Agnes Fay Morgan Research Award, the University of Kansas Graduate Student Mentoring Award, Purdue Distinguished Alumnus and the Higuchi Research Achievement Award in Biomedical Sciences. She was also recognized by the undergraduate students as a Center for Teaching Excellence Outstanding Teacher at KU and named an American Association of Colleges of Pharmacy Teacher of the Year. Dr. Lunte was the Editor-in-Chief of the RSC journal, Analytical Methods, from 2012-2017. She is a Fellow of the American Association of Pharmaceutical Scientists, American Chemical Society and the Royal Society of Chemistry. Dr. Lunte’s research interests include new methodologies for separation and detection of peptides, amino acids, neurotrans-mitters and pharmaceuticals in biological fluids.
Web Cast: This seminar is not web cast.